In Vitro Antibody-Dependent Cellular Cytotoxicity in Hematological cancer cells

We propose you to investigate the in vitro autologous or heterologous (with NK from healthy donor enrichment) cytotoxicity of your antibodies using cell lines or primary cells from patients with hematologic malignancies. For this experiment, we culture the primary cells with their bone marrow environment to best replicated the in vivo cytotoxicity of antibodies.

With the multi-parametric flow cytometry, we can simultaneously identify and analyze the effect of antibodies in various subpopulations of cells: normal and abnormal primary cells, T and B lymphocytes and NK cells. We can investigate the absolute number these cells in the different conditions. Additionally, we offer you the quantification of a target antigen in tumoral cells by Specific Antibody Binding Capacity assay (SABC).

  • Hematological cancer cell lines
  • Primary cells from patients with hematologic malignancies
  • Multiparameter flow cytometry
  • In vitro assessment of antibodies autologous cytotoxicity
  • In vitro assessment of antibodies heterologous cytotoxicity
  • A Final Report
  • Raw and Treated data